In conclusion, the presence of oxindole or pentacyclic alkaloids did not influence the antioxidant and antiinflammatory properties of cat's claw. These results indicate that while both species of cat's claw provide effective antioxidant and anti-inflammatory activities, U. Oral pretreatment for 3 d with UT protected against indomethacin-induced gastritis, and prevented TNFα mRNA expression and apoptosis. Non-alkaloid HPLC fractions from UT decreased LPS-induced TNFα and nitrite production in RAW 264.7 cells (P < 0.01) at a concentration range comparable to the parent botanical. The IC 50 value for inhibition of TNFa production was significantly (P < 0.01) higher for UT (14.1 ng/ml) vs UG (9.5 ng/ml), yet at concentrations that were considerable lower than that required for antioxidant activity. In each of the antioxidant assays tested, UG was more potent than UT (P UG. Apoptosis was assessed using the TUNEL technique and TNFα mRNA by in situ RT-PCR. Historically, cats claw has been used to. Anti-inflammatory activity was assessed in vitro by inhibition of TNFa and nitrite production from RAW 264.7 cells exposed to LPS (50 ng/ml) and in vivo using the indomethacin-induced gastritis model. Cats claw is a wood vine that grows wild in central and South America, particularly in the Amazon rainforest. Alkaloids and flavanols were determined using reversed-phase HPLC scavenging of 1,1-diphenyl-2-picrilhydrazyl (DPPH), hydroxyl radicals, and lipid peroxidation by spectrophotometry and TNFα production by ELISA. The purpose of this study was to characterize the antioxidative and antiinflammatory properties of cat's claw, Uncaria tomentosa (UT) and Uncaria guianensis (UG). Cat's claw is an herbal medicine from the Amazon that is used widely to treat inflammatory disorders.
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